Fluorescence:Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium.
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer
Article Snippet: .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and filter cubes for DAPI (32 mm, excitation 377/50, dichroic 409, emission 447/60; Nikon), mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Article Title: Identification of receptor-binding domains of Bacteroidales antibacterial pore-forming toxins
Article Snippet: Finally, 1 μl of cells was spotted onto mGAM low-melt agarose pads previously prepared using 65 μl Gene Frames (Thermo Scientific). .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and a filter cube for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Inverted Epifluorescence:Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium.
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer
Article Snippet: .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and filter cubes for DAPI (32 mm, excitation 377/50, dichroic 409, emission 447/60; Nikon), mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Article Title: Identification of receptor-binding domains of Bacteroidales antibacterial pore-forming toxins
Article Snippet: Finally, 1 μl of cells was spotted onto mGAM low-melt agarose pads previously prepared using 65 μl Gene Frames (Thermo Scientific). .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and a filter cube for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Fluorescence In Situ Hybridization:Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium.
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium
Article Snippet: .. Phase contrast and fluorescence images were taken with a Nikon Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100 × 1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH illuminator (Lumencor), a Prime95B camera (Photometrics), a temperature-controlled light-protected enclosure (Okolab), and filter-cubes for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Multi-dimensional image acquisition was managed using the NIS-Ar software (Nikon).
Article Title: Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer
Article Snippet: .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and filter cubes for DAPI (32 mm, excitation 377/50, dichroic 409, emission 447/60; Nikon), mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Article Title: Identification of receptor-binding domains of Bacteroidales antibacterial pore-forming toxins
Article Snippet: Finally, 1 μl of cells was spotted onto mGAM low-melt agarose pads previously prepared using 65 μl Gene Frames (Thermo Scientific). .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and a filter cube for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Microscopy:Article Title: Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer
Article Snippet: .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and filter cubes for DAPI (32 mm, excitation 377/50, dichroic 409, emission 447/60; Nikon), mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon), and GFP (32 mm, excitation 466/40, dichroic 495, emission 525/50; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
Article Title: Identification of receptor-binding domains of Bacteroidales antibacterial pore-forming toxins
Article Snippet: Finally, 1 μl of cells was spotted onto mGAM low-melt agarose pads previously prepared using 65 μl Gene Frames (Thermo Scientific). .. Phase contrast and fluorescence microscopy images were acquired on a Ti2-E fully motorized inverted epifluorescence microscope (Nikon) equipped with a CFI Plan Apochromat λ DM 100×1.45/0.13 mm Ph3 oil objective (Nikon), a Sola SEII FISH Illuminator (Lumencor), a Prime BSI camera (Photometrics), a temperature-controlled and light-protected enclosure (Okolab), and a filter cube for mCherry (32 mm, excitation 562/40, dichroic 593, emission 640/75; Nikon). .. Image acquisition was controlled by the NIS-Ar software (Nikon).
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